Calcium SignalingJames W. Putney, Jr. CRC Press, 1999年12月27日 - 416页 Arguably no other field of biological research embraces such a diverse array of experimental approaches as does the field of calcium signaling. Not only does it span virtually all conceptual and technical areas of molecular and cell biology, but a number of unique techniques, such as the use of permeabilized cells to study intracellular calcium metabolism, have evolved directly from calcium research. Calcium Signaling explores this fascinating area of research, covering the biophysical, molecular biological, and cell biological techniques involved in the study of calcium signaling. This work examines a broad range of topics-from techniques for imaging and measuring calcium in subcellular compartments, to subcellular fractionation and intracellular calcium stores, and patch clamp investigation of calcium channels. Filled with detailed illustrations, Calcium Signaling addresses those key methodological approaches that are useful to scientists and researchers investigating calcium function and regulation in many diverse areas of biological and medical research. |
目录
Chapter | 3 |
Molecular Mechanisms | 11 |
Conclusion | 37 |
Chapter 2 | 48 |
Acknowledgments | 63 |
Subcellular Fractionation | 73 |
References | 97 |
Subcellular Fractionation and Intracellular Calcium Stores 71 | 101 |
Functional Aspects | 183 |
Molecular Aspects | 203 |
Ryanodine Receptors | 227 |
Methods in Cyclic ADPRibose and NAADP Research | 249 |
297 | |
David Chin | 298 |
References | 314 |
Chapter 12 | 321 |
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常见术语和短语
3H]InSP activity adenophostin aequorin affinity assay BAPTA Biochem Biol Biophys buffer C-terminal Ca² Ca2+ binding Ca2+ concentration Ca2+ release Ca2+ signaling Ca2+ stores Ca2+ wave CADPR calcium channels calcium indicators calcium release calcium signaling calmodulin calreticulin calsequestrin CaM-binding cardiac cDNA Cell Calcium cell types cellular centrifugation cerebellar Chem confocal cytoplasmic cytosolic cytosolic Ca2+ domain endoplasmic reticulum enzymes expression Figure fluorescence fraction free Ca2+ function gene gradient homogenate ICRAC inactivation incubated inhibition inositol 1,4,5-trisphosphate inositol 1,4,5-trisphosphate receptor inositol trisphosphate InsP3 interaction intracellular Ca2+ intracellular calcium IP,R isoforms low-affinity mammalian measurements Mg2+ microsomes mitochondria Molecular NAADP neurons oocytes organelles peptide permeabilized phosphorylation Physiol pipette plasma membrane Pozzan protein kinase purified regulation release channel ryanodine receptor RyR1 RyR2 sarcoplasmic reticulum sea urchin sea urchin egg sequence SERCA SERCA2b skeletal muscle smooth muscle structure studies subcellular type-I vesicles Xenopus oocytes