Calcium Signaling

封面
James W. Putney, Jr.
CRC Press, 1999年12月27日 - 416页
Arguably no other field of biological research embraces such a diverse array of experimental approaches as does the field of calcium signaling. Not only does it span virtually all conceptual and technical areas of molecular and cell biology, but a number of unique techniques, such as the use of permeabilized cells to study intracellular calcium metabolism, have evolved directly from calcium research.
Calcium Signaling explores this fascinating area of research, covering the biophysical, molecular biological, and cell biological techniques involved in the study of calcium signaling. This work examines a broad range of topics-from techniques for imaging and measuring calcium in subcellular compartments, to subcellular fractionation and intracellular calcium stores, and patch clamp investigation of calcium channels.
Filled with detailed illustrations, Calcium Signaling addresses those key methodological approaches that are useful to scientists and researchers investigating calcium function and regulation in many diverse areas of biological and medical research.
 

目录

Chapter
3
Molecular Mechanisms
11
Conclusion
37
Chapter 2
48
Acknowledgments
63
Subcellular Fractionation
73
References
97
Subcellular Fractionation and Intracellular Calcium Stores 71
101
Functional Aspects
183
Molecular Aspects
203
Ryanodine Receptors
227
Methods in Cyclic ADPRibose and NAADP Research
249
Molecular Mechanisms by Which Ca2+Calmodulin Recognizes
297
David Chin
298
References
314
Chapter 12
321

Chapter 10
108
INTRODUCTION
111
PatchClamp Investigation of Calcium Channels
131
Xenopus Oocytes as a Tool in Calcium Signaling Research
157
Measuring Single Cell and Subcellular Ca2+ Signals
343
Methods for Studying Calcium Control of Secretion
365
Index
391
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